Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. What is the difference between liposomal glutathione and regular glutathione and what are the advantages Natural Field Co., Ltd. Ultimate Guide to Alpha Lipoic Acid Glutathione Absorption From Oral Mucosa & Skin Pigmentation CCID Dove Medical Press Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine