Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Let's talk Tylenol Paracetamol (acetaminophen) There are many side effects that most people don't realize. OTC does not mean it is side effect free, especially with chronic use. Even just 4 days of sagesteele @P_McCulloughMD While I dont know whether there is a connection between Tylenol and autism, I do believe that there is reason to STOP giving it to babies children. Glutathione is impacted by Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Sex differences in glutathione metabolism and acetaminophen toxicity